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Figure 2 | Journal of Translational Medicine

Figure 2

From: Detection and quantification of microRNA in cerebral microdialysate

Figure 2

High relative recovery of miRNA. Mean normalized cycle quantification (Cq) for miRNAs showing a high relative recovery during microdialysis in vitro (1A, 1B, 1C) and in vivo (2). 1A: Cq values of miRNAs after in vitro microdialysis on total RNA from human brain. Dark grey column, 20 kDa membrane; grey column, 100 kDa membrane; light grey column, undialyzed reference sample. 1B: Cq values of miRNAs after in vitro microdialysis on CSF from a neurologically healthy patient. Dark grey column, 100 kDa membrane; light grey column, undialyzed reference sample. 1C: Mean Cq values of miRNAs after in vitro microdialysis on CSF obtained by lumbar puncture in a neurologically healthy patient. Dark grey column, 20 kDa membrane; light grey column, undialyzed reference sample. Error bars show SD (N of identical aliquots = 4). 2: Mean Cq values of miRNAs in in vivo microdialysate and CSF obtained from patients with subarachnoid hemorrhage. Dark grey column, in vivo microdialysate, 20 kDa membrane; light grey column, CSF, external ventricular drain. Error bars show SD (N of aliquots for each = 4). The four samples of each material was taken from two different patients with SAH on Day 3 and 9.

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