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Figure 4 | Journal of Translational Medicine

Figure 4

From: Enhanced expression of cohesin loading factor NIPBL confers poor prognosis and chemotherapy resistance in non-small cell lung cancer

Figure 4

NIPBL knockdown enhanced cellular sensitivity to chemotherapy drugs, and increased chemotherapy drug-induced apoptosis in the H1299 cell line. H1299 cells were transfected with siNIPBL or non-targeting scramble siRNA for 24 h, and then treated with different concentrations of cisplatin, Gemcitabine hydrochlorid and paclitaxel for 24 h, seeded at the cell numbers indicated, cultured for 2 weeks. Clonogenic survival following treatment with: (A) cisplatin; (B) paclitaxel; (C) Gemcitabine hydrochlorid. Data represented mean ± SD for three replicate experiments. (D) H1299 cells were transfected with either siNIPBL or non-targeting scramble siRNA as indicated. After 48 h, cells were treated with lower concentration of cisplatin (0.5 ng/ml), Gemcitabine hydrochlorid (0.02 ng/ml) and paclitaxel (0.2 ng/ml) for 24 h. The cells were collected and stained with Annexin-V-FITC and PI. Shown were representative images of three independent experiments, values are expressed as a percentage of Annexin V positive versus total cells. (E) Apoptosis index were presented as mean ± SD of triplicate experiments. * P <0.05.

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