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Figure 4 | Journal of Translational Medicine

Figure 4

From: Development of genetically engineered iNKT cells expressing TCRs specific for the M. tuberculosis 38-kDa antigen

Figure 4

Proliferation, cytokine secretion and cytotoxicity of TCR gene-modified iNKT cells. (A) Proliferation of TCR gene-modified iNKT cells co-cultured with antigen-loaded or -unloaded DCs. (B) INF-γ, TNF-α and GrB secretion of TCR gene-modified iNKT cells at different E:T ratios (left panels) and time points (center panel). Compared with other control groups, TCR gene-modified iNKT cells exerted enhanced specific anti-TB antigen activities and these activities can be block by anti-HLA and anti-CD8 antibody (right panels). (C) Cytolytic activity of exogenous TCR gene-modified iNKT cells. UnTd + 38-kDa (untransduced iNKT cells + 38-kDa antigen-loaded DCs); EmTd + 38-kDa (empty vector- transduced iNKT cells + 38-kDa antigen-loaded DCs); Td + α-Galcer (TCR gene-modified iNKT cells + α-Gal-cer-loaded DCs); Td + OVA (TCR gene-modified iNKT cells + OVA-loaded DCs); Td + ESAT-6 (TCR gene-modified iNKT cells + ESAT-6 antigen -loaded DCs); Td + 38-kDa (TCR gene-modified iNKT cells + 38-kDa antigen-loaded DCs). *P < 0.001 compared with UnTd + 38-kDa group.

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