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Figure 3 | Journal of Translational Medicine

Figure 3

From: Osteoinduction and proliferation of bone-marrow stromal cells in three-dimensional poly (ε-caprolactone)/ hydroxyapatite/collagen scaffolds

Figure 3

Osteogenic differentiation of BMSCs growing on scaffolds. The mRNA expression of bone tissue specific markers, including Runx2 (A), ALP (B), Osterix (C), Osteocalcin (D), RANKL (E) were evaluated during the in vitro culturing period of up to 28 days to assess osteogenic differentiation of BMSCs by RT-qPCR at different time points. All data were normalized to the mRNA expression of the corresponding marker at day 0 (cell seeding day). Data indicate the mean relative values calculated from six independent experiments (±S.E.). Statistically significant differences with P < 0.05 were considered significant (*P < 0.05). F, shows a representative protein expression (as indicated) of cell lysates cultured on PCL scaffolds only, PCL scaffolds with poly-lysine coating, PCL scaffolds with HAp, PCL scaffolds with HAp and 30 mg/ml collagen coating, PCL scaffolds with 10 mg/ml collagen coating, PCL scaffolds with 30 mg/ml collagen coating after 28 days of cell culturing.

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