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Figure 4 | Journal of Translational Medicine

Figure 4

From: Establishment of a patient-derived orthotopic osteosarcoma mouse model

Figure 4

Establishment of the primary tumor cell line OS-RH-2011/5. The original tumor tissue was collected and directly cultivated into medium (P1*). After 26 days, cells were split and 3.75 × 106 cells were injected subcutaneously into the left flank of six athymic BALB/c Nu/Nu mice. After three weeks, four of six mice developed a tumor mass, which was removed when reaching a volume of 1.500 mm3. Tumor cells were then passaged in culture (P2*), split after 20 days and again injected into the flank of six mice. After two weeks, all mice developed a tumor mass, which was removed and re-cultured (P3*)

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