Skip to main content
Figure 3 | Journal of Translational Medicine

Figure 3

From: Defining novel parameters for the optimal priming and expansion of minor histocompatibility antigen-specific T cells in culture

Figure 3

Expansion of MiHA-specific T-cell lines in REP. (A) Kinetics of culture growth as represented by the fold increase in cell count from the number of IFNγ-secreting cells seeded at the beginning of the REP step; n = 4. (B) left: one representative of the CD8+ HA-1+ cells generated over time analyzed by flow cytometry; right: variation of CD8+ HA-1+ over time for each donor. (C) Percentage of specific lysis measured with a Cr51 release assay of autologous or allogenic HLA-A0201 HA-1Neg cells pulsed or not with HA-1 peptide; n = 4. *, P < 0.05; **, P < 0.01.

Back to article page