Induction of terminal differentiation marker expression in coculture over time. (A) CD45RO, CD62L and CCR7 expression on HA-1 multimer-positive cells. (B) left: representative plots and right: combination of PD-1 and KLRG-1 expression over time on HA-1+ and HA-1- CD8+ T cells; n = 4. Comparison of (C) PD-1 and (D) KLRG-1 expression between the multimer-positive and negative CD8+ T-cell fractions within the cultures at different time points ; n = 4, data represented as average ± SEM. *, P < 0.05.