Figure 5From: A novel method for downstream characterization of breast cancer circulating tumor cells following CellSearch isolationImmunostaining of metastatic breast cancer patient blood samples. Representative images of positive ERα and HER2 staining in clinical samples. Row A, from left to right: DAPI counterstain (fluorescent blue), cytokeratins 8, 18, and 19 (CK) stained with Phycoerythrin (red), estrogen receptor (ERα) stained with AlexaFluor 488 (green), and a composite of all channels. This patient sample (no. 4, see Table 3) was collected prior to initiation of therapy, illustrating two clustered ERα+ CTCs, adjacent to a solitary leukocyte located in the lower left corner. This patient was diagnosed with an ERα+ metastasis. Row B, from left to right: DAPI counterstain (fluorescent blue), HER2 stained with Liquid Permanent Red (red). This patient sample (no. 1, see Table 3) was obtained following 6 months of chemotherapy, illustrating HER2+ CTCs identified by combination of fluorescence and bright-field microscopy. This patient was diagnosed with a HER2− primary tumor and HER2− metastasis.Back to article page