Figure 5
Ectopic expression of AFAP1-AS1 promoted proliferation, migration, and invasion of pancreatic cancer cell. (a) AFAP1-AS1 expression level was confirmed by qPCR 48 hours after transfection. (b) The effect of AFAP1-AS1 overexpression on PANC-1 cell proliferation was determined by MTT assays. (c) Representative images of transwell assay after AFAP1-AS1 ectopic expression n in PANC-1 cells. (d) Analysis of OD (570 nm) absorbance of crystal violet-stained cells in migration assay and invasion assay. (e) Relative mRNA expression levels of EMT-related genes (normalized to β-actin) in PDAC cells after AFAP1-AS1 knockdown were determined by RT-qPCR. (f) Western blot analysis of E-cadherin, N-cadherin, Vimentin, Slug, and Snial1. Data are represented as the mean ± s.d. from three independent experiments. Vector control denotes cells transfected with empty vector. ***: P < 0.01, Student’s t-test.