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Figure 4 | Journal of Translational Medicine

Figure 4

From: High expression of AFAP1-AS1 is associated with poor survival and short-term recurrence in pancreatic ductal adenocarcinoma

Figure 4

AFAP1-AS1 knockdown inhibits migration and invasion of PDAC cells. (a) Representative images of transwell assay after AFAP1-AS1 knockdown in PDAC cell line MIAPaCa-2 and SW1990. (b) Analysis of OD (570 nm) absorbance of crystal violet-stained cells in transwell assay. (c) Representative images of Matrigel invasion assay after AFAP1-AS1 knockdown in PDAC cell line MIAPaCa-2 and SW1990. (d) Analysis of OD (570 nm) absorbance of crystal violet-stained cells in Matrigel invasion assay. (e-f) Relative mRNA expression levels of EMT-related genes (normalized to β-actin) in PDAC cells after AFAP1-AS1 knockdown were determined by RT-qPCR. (g) Western blot analysis of E-cadherin, N-cadherin, and Vimentin. Data are represented as the mean ± s.d. from three independent experiments, shControl denotes shRNA having no homology to any known mammalian genes as a negative control. ***: P < 0.01, Student’s t-test.

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