Figure 3
AFAP1-AS1 knockdown attenuates PDAC cell proliferation. (a) AFAP1-AS1 expression level was confirmed by RT-qPCR. (b-c) The effect of AFAP1-AS1 knockdown on cancer cell line proliferation was determined by MTT assays. (d-e) The effect of AFAP1-AS1 knockdown on the cell cycle was measured by flow cytometry. Data are represented as the mean ± s.d. from three independent experiments; shControl denotes shRNA having no homology to any known mammalian genes as a negative control. ***: P < 0.01, Student’s t-test.