Figure 1

AFAP1-AS1 expression in clinical pancreatic ductal adenocarcinoma specimens and cancer cell lines. (a) Heat map representing the top 50 up-regulated long noncoding RNAs in tissue from eight cases of pancreatic ductal adenocarcinoma (PDAC) compared with tissue from four cases of chronic pancreatitis (CP). Group 1 indicates the most up-regulated lncRNAs in PDAC tissues from patients with diabetes, and group 2 indicated the most up-regulated lncRNAs in PDAC tissues from patients without diabetes. (b) The AFAP1-AS1 expression levels in PDAC cell lines were determined via RT-qPCR, and β-actin was used as internal control. Data are represented as the mean ± s.d. from three independent experiments. ***: P < 0.01, Student’s t-test. (c) AFAP1-AS1 expression levels in 90 paired PDAC tissues and their adjacent noncancerous tissues were examined via RT-qPCR. The relative AFAP1-AS1 mRNA level was normalized to β-actin.