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Figure 4 | Journal of Translational Medicine

Figure 4

From: Estradiol induces HOTAIR levels via GPER-mediated miR-148a inhibition in breast cancer

Figure 4

miR-148a targets HOTAIR in breast cancer cells. A): HOTAIR has binding sequences for miR-148a. B): The level of miR-148 was associated with the HOTAIR mRNA level in the PBMCs from the breast cancer patients. Spearman’s correlation coefficient [rs] = r2 = 0.6492, p = 0.009. C): The level of miR-148 was associated with the HOTAIR mRNA level in the tumor tissues from the breast cancer patients. Spearman’s correlation coefficient [rs] = r2 = 0.6251, p = 0.004. D: The MDA-MB-231 cells were transfected with negative miRNA, miR-148a mimics and miR-148a for 24 h, and then the level of miR-148a was assessed using qRT-PCR. E): The luciferase activities of the MDA-MB-231 and BT549 cells were measured after co-transfection with PGL3-HOTAIR and miR-148a or its inhibitor for 24 h. The results are shown as mean ± S.E. from three representative independent experiments. **P <0.01 vs. control. F): The luciferase activity of the MDA-MB-231 and BT549 cells was measured after co-transfection with the indicated mutated PGL3-mHOTAIR constructs and miR-148a or its inhibitor for 24 h. The results are shown as mean ± S.E. from three representative independent experiments. G): The MDA-MB-231 and BT549 cells were transfected with miR-148a mimics before E2 treatment. Then the transfected cells were treated with 10 nM E2 for 24 h, and the expression of HOTAIR was checked with real-time PCR. The results are shown as mean ± S.E. from three representative independent experiments. **p <0.01 vs control, #p < 0.05 vs. E2 alone.

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