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Figure 4 | Journal of Translational Medicine

Figure 4

From: Comprehensive molecular characterization of human adipocytes reveals a transient brown phenotype

Figure 4

Human SGBS adipocytes display features of white and brown adipocytes respectively. (a) Oil Red staining was carried out as described above and the presence of stained lipid droplets at D14, D21 and D28 was assessed at a magnification of 20×. (b and c) Premature and differentiated SGBS cells were co-stained with mitotracker (green) to test the abundance of mitochondria, lipidtox (red, in b) for neutral lipid droplets or anti-UCP1 antibodies (red, in c) and DAPI (blue) to visualize nuclei. Single-channel images were overlayed and processed by Photoshop software. All scale bars are reported.

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