Differentiated SW872 adipocytes depict a high abundance of lipid droplets but no UCP1 expression. (a) Oil Red staining was carried out as described above and the presence of stained lipid droplets at D7 was assessed at a magnification of 20×. (b and c) Premature and differentiated SW872 cells were co-stained with mitotracker (green) to test the abundance of mitochondria, lipidtox (red, in b) for neutral lipid droplets or anti-UCP1 antibodies (red, in c) and DAPI (blue) to visualize nuclei. Single-channel images were overlayed and processed by Photoshop software. All scale bars are reported.