Figure 2

Differentiated PAZ6 cells overexpress adipokines and brown adipocyte markers. PAZ6 cells were cultured in monolayers and differentiated for 14 days. RNA was isolated using the Qiagen lipid tissue mini kit and cDNA synthesis was carried out. Quantitative real-time PCR was performed by SYBR green detection method and values are reported as RQ normalized referring to the relative quantification compared to HPRT expression and normalized for D0 baseline expression levels for each gene. All experiments were done in triplicates and data are derived from at least three independent experiments. (* p <0.01, ** p < 0.001).