Figure 6

The effect of cytokines on CD25 receptor expression on NK cell subsets. a) The percentage and mean fluorescence intensity (MFI) of CD25 receptor increase significantly (*p < 0.05, Wilcoxon signed rank test) on CD3⁻CD56⁺NK cells and their CD3⁻CD56^dim+ and CD3⁻CD56^bright+ subsets in HC and MM patients after 18 h in vitro treatment with IL-12 and IL-18 in combination. Results are obtained by Flow cytometry; b) HC and MM patients in M1a + M1b group have significantly (*p < 0.05, Mann–Whitney exact test) higher increase in CD25 receptor expression on NK cells after in vitro treatment with combination of IL-12 and IL-18 compared to the increase in M1c group. Results are expressed in indexes calculated as the value of CD25 receptor expression after combined IL-12 and IL-18 treatment of each HC or MM patient devides with the value of this receptor expression after treatment in medium alone.