Effects of extracts and extract fractions on PLNs in BALB/c mice. (A) WI and CI in the PLNs of treated mice. Compared with the untreated side of each group, the WI and CI values were significantly increased by EAE, NBE and AEE (WI > 2 and CI > 5), whereas AE showed no marked difference. The data are presented as the means ± SD from 10 mice per group. ★P < 0.05 and ★★P < 0.01 vs. the untreated side. (B) Photographs of the PLNs in the cell suspensions from the treated side. DF, AEE, EAE and NBE caused elevations in the number of PLNs. The photographs were taken using a CountstarIC1000 automatic cell counting instrument. a, DF group; b, AEE group; c, EAE group; d, NBE group; e, AE group; f, normal group.