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Table 1 Serum and plasma levels of cytokines and chemokines for each condition measured

From: Diurnal rhythms of serum and plasma cytokine profiles in healthy elderly individuals assessed using membrane based multiplexed immunoassay

  

Serum AM (n = 22)

Serum PM (n = 22)

Plasma AM (n = 22)

Plasma PM (n = 22)

High MFI

RANTES

7.9 ± 1.4

7.8 ± 1.1

8.5 ± 1.1

10.3 ± 1.2

Sicam-1

6.8 ± 1.1

6.7 ± 0.8

7.6 ± 1.1

9.0 ± 1.0

Serpin E1

6.0 ± 1.0

6.6 ± 0.9

6.1 ± 0.9

6.5 ± 0.6

MIF ††

3.4 ± 0.5

3.5 ± 0.4

4.0 ± 0.5

5.1 ± 0.5***

Medium MFI

IL-16 †††

1.2 ± 0.2

1.2 ± 0.1

1.2 ± 0.1

1.7 ± 0.1***

IL-1ra †††

1.2 ± 0.2

1.3 ± 0.1

1.2 ± 0.1

1.7 ± 0.1***

C5a †††

1.1 ± 0.1

1.1 ± 0.1

1.1 ± 0.1

1.6 ± 0.1***

GROa †††

1.1 ± 0.1

1.1 ± 0.1

1.0 ± 0.1

1.5 ± 0.1***

GM-CSF †††

0.9 ± 0.0

1.0 ± 0.1

1.0 ± 0.0

1.5 ± 0.1***

SDF-1 †††

0.9 ± 0.1

1.0 ± 0.1

1.0 ± 0.0

1.5 ± 0.1***

CD40 Ligand †††

1.0 ± 0.1

1.1 ± 0.1

0.9 ± 0.0

1.3 ± 0.1***

Low MFI

Strem-1 ††

0.9 ± 0.0

1.0 ± 0.1

1.0 ± 0.0

1.5 ± 0.1***

IP-10 †††

0.8 ± 0.0

0.9 ± 0.1

0.9 ± 0.0

1.4 ± 0.1***

TNF-a ††

0.9 ± 0.0

0.9 ± 0.1

0.9 ± 0.0

1.4 ± 0.1***

IL-2 †††

0.8 ± 0.0

0.9 ± 0.1

0.9 ± 0.0

1.4 ± 0.1***

IL-8 †††

0.8 ± 0.0

0.8 ± 0.0

0.9 ± 0.0

1.3 ± 0.1***

MIP-1ß ††

0.8 ± 0.0

0.8 ± 0.0

1.0 ± 0.1

1.3 ± 0.0***

IL-1ß †††

0.8 ± 0.0

0.8 ± 0.0

0.9 ± 0.0

1.3 ± 0.0***

G-CSF †††

0.9 ± 0.1

0.9 ± 0.1

0.9 ± 0.1

1.4 ± 0.1***

I-309 /CCL1 †††

0.9 ± 0.2

0.9 ± 0.1

0.9 ± 0.1

1.4 ± 0.1***

INF-g †††

0.7 ± 0.0

0.7 ± 0.0

0.8 ± 0.0

1.2 ± 0.0***

IL-1a †††

0.7 ± 0.0

0.7 ± 0.0

0.8 ± 0.0

1.2 ± 0.0***

IL-4 †††

0.7 ± 0.0

0.8 ± 0.0#

0.8 ± 0.0

1.2 ± 0.0***

IL-5 †††

0.7 ± 0.0

0.7 ± 0.0

0.8 ± 0.0

1.2 ± 0.0***

IL-6 †††

0.8 ± 0.0

0.8 ± 0.0

0.9 ± 0.0

1.3 ± 0.0***

IL-10 †††

0.7 ± 0.0

0.7 ± 0.0

0.8 ± 0.0

1.1 ± 0.0***

IL-12 p70 †††

0.8 ± 0.0

0.8 ± 0.0

0.9 ± 0.0

1.3 ± 0.1***

IL-13 †††

1.0 ± 0.2

0.9 ± 0.1

0.9 ± 0.1

1.3 ± 0.1***

IL-17 †††

0.8 ± 0.0

0.8 ± 0.0

0.9 ± 0.0

1.3 ± 0.0***

IL-17E †††

0.9 ± 0.1

0.9 ± 0.1

0.9 ± 0.1

1.4 ± 0.1***

IL-23 †††

0.7 ± 0.0

0.8 ± 0.0##

0.8 ± 0.0

1.2 ± 0.0***

IL-27 †††

0.7 ± 0.0

0.7 ± 0.0#

0.8 ± 0.0

1.1 ± 0.0***

IL-32a †††

0.7 ± 0.0

0.7 ± 0.0

0.8 ± 0.0

1.1 ± 0.0***

I-TAC †††

0.7 ± 0.0

0.8 ± 0.0#

0.8 ± 0.0

1.2 ± 0.0***

MCP-1 †††

0.7 ± 0.0

0.8 ± 0.0#

0.8 ± 0.0

1.2 ± 0.0***

MIP-1a †††

0.7 ± 0.0

0.7 ± 0.0#

0.8 ± 0.0

1.2 ± 0.0***

  1. NOTE. #significant at p < 0.05, ##or ††significant at p < 0.01, *** or †††significant at p < 0.001.
  2. MFI = Median Fluorescence Intensity.
  3. The table is divided in three parts: the “high” intensities (on top) represent the cytokines that are more abundant in the circulation. The “medium” intensities (in the middle) represent the cytokines with MFI values above the threshold for at least two conditions. The “low” intensities (on the bottom) represent all the cytokines with MFI values below the threshold. Values are expressed in MFI ± SEM. Differences compared to “Serum AM” are marked with #and the ones compared to “Plasma AM” with *. The statistical analysis with two-way ANOVA further demonstrated that there is a “significant interaction” between serum and plasma. Therefore, the increase between AM and PM values is dependent on the type of matrix (serum or plasma) that is used. This interaction is indicated by for each corresponding cytokine/chemokine that is found to show a “significant interaction”.