Figure 4

Amplification and over-expression of HER2 fusions in gastric tumors. A. Histological characterization of human gastric carcinomas. Representative images from H&E staining of GC196 and GC334 primary tumors. B. ZNF207-HER2 gene fusion in primary tumor GC196. Representative images of break-apart FISH on GC196 shows normal copy of HER2 N-terminal (green signals), coexistence of amplified HER2 C-terminal (red signals) and amplified ZNF207 (gold signals). Red and green signals represent C-terminal (HER2-C-SR) and N-terminal (HER2-N-SG) of HER2 gene, respectively; gold signals represent whole length of ZNF207 gene (ZNF207-WL-SGo); aqua signal represent CEP17 as internal control. Cell nuclei were counterstained with DAPI. C. NOS2-HER2 gene fusion in tumor GC334 primary tumor. Representative images of break-apart FISH on GC334 show NOS2-HER2 fusion with heterogeneity. The upper lane showed tumor cells with both HER2 N-terminal (green signals) and C-terminal (red signals) amplified and normal copy number of NOS2 (gold signals). The lower lane showed tumor cells with normal copy of HER2 N-terminal (green signals), coexistence of amplified HER2 C-terminal (red signals) and amplified NOS2 (gold signals). Red and green signals represent C-terminal and N-terminal of HER2 gene; gold signals represent NOS2 gene; aqua signal represent CEP17 as internal control. Cell nuclei were counterstained with DAPI. D. IHC analysis of HER2. Representative images showed HER-2 IHC strong membrane staining (+++) on GC196 and GC334 primary tumors.