Figure 3

Abundance of selected neutrophil proteins in UP samples. Thirteen proteins listed in the legend on the far right are neutrophil granule proteins. Three other proteins are fibrinogen-β (FGB; implicated in coagulation), hemoglobin α-subunit (HBA1; indicative of vascular injury), and uromodulin (UMOD; abundant in urine of healthy donors). The samples SA_112 and PM_20 represented UTIs caused by S. aureus (SA) and P. mirabilis (PM), respectively. The profile of LG_23 (Lactobacillus) indicated the lack of inflammation, and represented urethral colonization, possibly also minor vaginal contamination of the urine sample. KP_10 (K. pneumoniae) appeared to represent vaginal infection as vaginal bleeding was clinically diagnosed for the patient. The protein profiles of EC_13 (UPEC) and KP_55 suggested the near-absence of inflammation, and therefore most likely urethral colonization. Proteins were quantified using the iBAQ method, in each case divided by the summed iBAQ values for the entire UP proteome.