Figure 2

Protein profiles showing quantitative contributions of neutrophils, the complement system, and erythrocytes to the total proteome of urinary pellet samples. The graph displays the summed protein abundances, relative to the total proteome, for three biological protein categories. The x-axis lists the identifiers of the UP samples associated with 110 human subjects. The three categories represent proteins produced by activated neutrophils (BLUE), proteins highly expressed in erythrocytes and released upon vascular injury (GREEN), and proteins associated with complement system activity and coagulation (RED). The method used for quantification of all proteins is the iBAQ method in the MaxQuant software tool. The order of samples is based on neutrophil protein abundance, decreasing from left to right. To allow direct comparisons for the assessment of inflammation, the score for the LE assay was included in the graphic above each bar representing a sample. Underneath the x-axis, an additional bar depicts which samples were associated with the ID of a pathogen causing UTI (ORANGE color segments), commensal bacteria (GREEN color segments) or lack of bacterial IDs (no color).