Figure 3

Gene expression analysis of IFN-DCs. a. Unsupervised Hierarchical Clustering of samples using the whole dataset. Monocyte, IFN-DCs and prior to cryopreservation DCs are shown by red, green and blue bars, respectively; b) Heatmap of the average corrected expression levels of the 5725 genes differentially expressed between IFN-DCs and monocytes with a p-value < 0.001. Genes are in rows and samples in columns. Monocyte, IFN-DCs and prior to cryopreservation DCs are shown by red, green and blue bars, respectively; c) Gene Ontology Analysis of up-regulated genes in IFN-DCs vs monocytes (p-value < 0.001 and ratio >3). The plot show for each GO “biological function” term the enrichment among genes up-regulated in IFN-DCs expressed as –log10(p-value). Enrichment p-values were calculated through hypergeometric test. Statistical significance threshold for hypergeometric test was set to 0.05 (i.e., −log10(p-value) > 1.3 were statistically significant).