Figure 5

Vorinostat treatment and preconditioning inhibits anchorage-independent OS cell growth. A/B: mHOS cells were treated with either 2 μM vorinostat or DMSO alone and allowed to grow for 4 days in AI conditions. Vorinostat treatment decreased the number of spheres (A), and resulted in a statistically significant decrease in cell viability (B). C/D: Adherent mHOS cells were pretreated for 12 days with either 2 μM vorinostat or DMSO alone and subsequently plated and allowed to grow for 4 days in AI conditions in the absence of drug. Vorinostat pretreatment decreased the number of spheres (C) and resulted in a significant decrease in cell viability (D). Asterisks indicate statistical significance (*p < 0.05, ***p < 0.001).