Figure 4

miR-125b was implicated in the effect of GLPS on intratumoral Tregs. (A) The expression of many miRNAs including miR-126, miR-155, miR-146a, miR-224, miR-150 and miR-125b was measured in Tregs from hepatoma-bearing mice injected with GLPS. **P < 0.01, indicate significant differences from Tregs isolated from hepatoma-bearing mice injected with 0 mg/kg GLPS. (B) The expression of miR-125b was determined in naïve T cells under iTreg polarizing condition with various concentrations of the GLPS for 96 h. **P < 0.01, indicate significant differences from 0 μg/ml GLPS treatment. The mRNA (C) and protein (D) levels of FoxP3 were measured in naïve T cells under iTreg polarizing condition with various concentrations of the GLPS for 96 h. **P < 0.01, indicate significant differences from 0 μg/ml GLPS treatment. After transfected with miR-125b inhibitor for 24 h, naïve T cells under iTreg polarizing condition were treated with GLPS (100 μg/ml) for 96 h. Then, the protein levels of FoxP3 (E) and Notch1 (F), and the mRNA level of Notch1 (G) were determined. **P < 0.01, indicate significant differences from the respective control groups. **P < 0.01, indicate significant differences from the respective control groups. (H) After transfected with miR-125b inhibitor for 24 h, naïve T cells under iTreg polarizing condition were treated with GLPS (100 μg/ml) or GLPS (100 μg/ml) + curcumin (30 μmol/L). Then, the FoxP3 protein level was determined.