Figure 6
FH535 acts as a potential radiosensitizer in KYSE-150R cells. (A) Clonogenic survival in FH535 (20 μM) treated KYSE-150R cells after irradiation. The data points show mean survival fraction from 3 individual experiments. (B) CCK8 proliferation assays were performed on the KYSE-150R cells treated with increasing doses of FH535 (0.0-20 μM) over 24 hours. Graph represents the average cell proliferation in percentage of 6 replicates with standard deviation represented by error bars. (C) Comet assay of DNA damage response. The cells were cultured for the given periods and then subject to agarose gel electrophoresis. The photographs show the momentum of the nuclear DNA movement. (D) Summary data of momentum of cellular DNA. Data shown from three independent experiments. *P < 0.05.