Figure 5From: FH535 increases the radiosensitivity and reverses epithelial-to-mesenchymal transition of radioresistant esophageal cancer cell line KYSE-150R EMT suppress in KYSE-150R cell line with FH535. (A) The effect of FH535 on EMT target genes expression determined by RT-PCR. All samples were normalized to the signal generated from housekeeping gene (β-actin). (B) Western blot analysis of EMT protein levels treated with FH535 for 24 h, GAPDH was used as normalization control. (C) Immunofluorescence analysis showed protein levels of E-cadherin and Vimentin. Nuclei were counterstained with DAPI (blue). The images were zoomed in 400×.Back to article page