Repression of nuclear translocation of β-catenin with FH535. (A) Western blotting analysis determined Nuclear and cytoplasmic expression of β-catenin in the radioresistant KYSE-150R cells treated with or without FH535. β-actin was the loading control. Data are representative of at least 3 independent experiments. (B) Expression of the β-catenin detected by immunofluorescence analysis. Nuclei were counterstained with DAPI (blue). The images were zoomed in 400×.