Figure 3

Activation of Wnt pathway constitutively in radioresistant esophageal cancer cell. (A) mRNA expression of Wnt pathway protein was measured using RT-PCR. Graph represents the mRNA fold-regulation values of radioresistant KYSE-150R cells relative to parental cells, normalized against housekeeping gene (GAPDH) with standard deviation of triplicate experiments represented by error bars. (B) Protein level confirmation of activated Wnt signaling markers using western blot. GAPDH was the loading control. Data are representative of at least 3 independent experiments.