Figure 2

Let-7a inhibited the protein expression of HMGA2 by binding to its 3′UTR. (A) RT-qPCR of let-7a expression in NPC cell lines with different degrees of differentiation and metastasis ability. (B) CNE-2 and 5-8F cells transfected with the let-7a mimics showed significantly increased let-7a expression compared to controls. (C) Position of the let-7a target site in 3′UTR of HMGA2 mRNA predicted by TargetScan. (D) Relative luciferase activity of CNE-2 and 5-8F cells after co-transfection with wild type (WT) or mutant (MUT) HMGA2 3′UTR reporter genes and let-7a mimics or control. (E-F) Protein and mRNA expression levels of HMGA2 are tested after let-7a transfected in CNE-2 and 5-8F by western blotting and RT-qPCR. *, P < 0.001 compared with control.