Flow cytometric analyses in uninfected PM1 cells. ND-4043 is used to compete with binding of 4 anti CXCR4 (A) and 6 anti CD4 (B) moAbs. Incubation with ND-4043 was carried out in PBS plus 2% (A) or 1% (B) of heat-inactivated FCS. A. ND-4043 was used at three concentrations (30, 10 and 3 μM) and also AMD3100 (3, 0.3 and 0.03 μM). B. ND-4043 was used at 30 μM. DMSO refers to control cells stained with the moAbs but pre-incubated only with DMSO at 0.2%, corresponding to the concentration present in 30 μM of the compound; uns: unstained cells. A color coded legend is displayed within the figure.