Figure 3

Effect of different concentrations of the six compounds plus AMD3100 and maraviroc on HIV-1-mediated fusion (A) and cytotoxicity (B). Fusion assays were carried out in complete medium plus 2.5% of heat-inactivated FCS with SupT1-IIIB (X4) or PM1-BaL (R5) as donor cells and TZM-bl as target cells (left panel X4, right panel R5). Viral fusion data, expressed as OD, are presented as mean ± SD of three replicates on the x axes, whereas concentrations of tested compounds are reported on the y axes. B. MTT assay on day 4 cultures of TZM-bl not infected with vaccinia virus, in the presence or absence of different concentrations of the six compounds. Data are expressed as OD and represent mean ± SD of three replicates. Control TZM-bl were incubated with DMSO at 0.2% corresponding to the concentration present in 30 μM of the compounds.