Figure 3

Specific siRNA of FAK and Pyk2 inhibited hypoxia-induced U251 glioma cells migration and invasion. (A) Specific siRNA significantly reduced the total and phosphorylation level of FAK and Pyk2. (B) Effect of FAK and Pyk2 siRNA on migration of U251 glioma cells using a transwell assay. The migratory cells were stained by 0.1% crystal violet (upper). The migratory cell number of control was expressed as 100% (lower) *p <0.05, **p <0.01. (C) Effect of FAK and Pyk2 siRNA on invasion of U251 glioma cells by a matrigel-precoated transwell assay. The invasive cells were stained by 0.1% crystal violet (upper). The invasive cell number of control was expressed as 100% (lower). *p <0.05, **p <0.01 vs control.