Figure 1

Effect of melatonin on the migration and invasion of U251 glioma cells under hypoxia. (A) The migratory ability of U251 glioma cells treated with different concentration of melatonin was evaluated by wound healing assay. The representative images at 0 hour and 24 hours post-wounding are shown at 10 × magnification. (B) U251 glioma cell migration was analyzed using transwell assay after melatonin treatment for 24 hours. The migratory cells were stained by 0.1% crystal violet (upper). The migratory cell number exposed to hypoxia alone was expressed as 100% (lower). (C) U251 glioma cell invasion was detected by matrigel-precoated transwell assay after melatonin treatment for 24 hours. The invasive cells were stained by 0.1% crystal violet (upper). The invasive cell number exposed to hypoxia alone was expressed as 100% (lower). The data presented the mean of three independent experiments. *p < 0.05, **p < 0.01.