Figure 3

GLI2 induces cell cycle arrest in leukemic progenitors. a. Both the lentiviral GLI2 wild-type (GLI2) and transactivation domain deleted (GLI2 ΔTAD) expression constructs contain an N-terminus Flag epitope tag. The scheme depicts both wild type and mutant GLI2. GLI2 ΔTAD mutant harbors a deletion of the transactivation domain reported in [48]. b. Western blot of 293A cells transduced with GLI2 and immunoprecipitated using flag epitope. c. FACS based cell cycle analysis of patient samples (CD45+) that had either been transduced with vector, wild type (GLI2), or mutant (GLI2). d-e. Spearman correlation analysis comparing GLI2 expression levels, determined by quantitative PCR, and percent of cells in G0 after lentivital GLI2 or GLI2 ΔTAD compared with backbone vector controls.