Figure 4

Berberine attenuated ox-LDL-induced inflammation through the induction of autophagy in J774A.1 cells. (A) Representative blots of LC3 and SQSTM1/p62 in ox-LDL stimulated J774A.1 cells treated with berberine in the presence or absence of 30 μM CQ. (B) Bar graphs showed the quantification of the indicated proteins. (C) Representative flow cytometry scatter plot of J774A.1 cells after treatment. Experiments were repeated at least three times. (D) The levels of MIP-1α, RANTES and IL-10 in the culture media from ox-LDL-stimulated J774A.1 cells were detected via Aimplex. (n = 4) (E) Cells were double stained by LC3 and DAPI and examined by fluorescence microscopy (200×, 400×). (F) Cells were double stained by SQSTM1/p62 and DAPI and examined by fluorescence microscopy (200×, 400×). (G) Representative TEM images of J774A.1 cells Arrows indicated the autophagosomes (30.0 kx, 15.0 kx, 5.0 kx). **P < 0.01 V.S. control group, *P < 0.05 V.S. control group; ##P < 0.01 V.S. ox-LDL group; $$P < 0.01 V.S. BBR group, $P < 0.05 V.S. BBR group.