Figure 2
Endogenous LC3 in the lysates was recognized by immunoblotting with an anti-LC3 antibody. LC3-I, soluble form of LC3; LC3II, membrane-bound form of LC3. (A) Representative blots of LC3Iand LC3II in J774a.1 cells incubated with 100 mg/L ox-LDL for different time points (0.75, 1.5, 3, 6, 12 and 24 h) using Western blot analysis. (B) Bar graphs showed the LC3II/ LC3Iratio quantified by Image Lab 3.0 software. (C) Representative blots of SQSTM1 in the cells incubated by ox-LDL in the presence or absence of 30 μM CQ. (D) Bar graphs showed the band value of SQSTM1/p62. (E) Representative Flow cytometry scatter plot of J774A.1 cells after treatment with ox-LDL and CQ. (F) The levels of RANTES in the culture medium were detected via AimPlex. Each experiment was repeated at least three times. **P < 0.01 V.S. control group; ##P < 0.01 V.S. 0.75 h, 1.5 h, 3 h and 6 h groups; $P < 0.05 V.S. ox-LDL group.