Figure 6

HIF2α-mediated ESC cardiac differentiation is β-catenin-dependent. (A) TOP Flash assay in PB- or PB-HIF2α- EBs at day 4. Data represent the mean ± s.d. of three biological replicates. **p < 0.01 vs PB-EBs. (B) Western blot analysis of β-catenin at day 4 PB- or PB-HIF2α- EBs. (C) TOP Flash assay of ESCs treated with the indicated conditions for 12 h. Data represent the mean ± s.d. of three biological replicates. **p < 0.01. NT: No treatment. (D) The percentages of beating EBs derived from PB- or PB-HIF2α- ESCs in the presence or absence of 2 μm XAV939 or 2 μm IWR1. Data represent the mean ± s.d. of three biological replicates. **p < 0.01. (E) qRT-PCR analysis of Gata4, Nkx2.5 and Mef2C in PB- or PB-HIF2α- EBs in the presence or absence of 2 μm XAV939 or 2 μm IWR1. Data represent the mean ± s.d. of three biological replicates. **p < 0.01 vs PB.