Figure 4

Knockdown of HIF2α impairs the ability of ESCs for cardiac differentiation. (A) qRT-PCR analysis of HIF2α mRNA levels in mouse ESCs stably transfected with HIF2α shRNA. The transcript level was normalized against scramble shRNA controls. Data represent the mean ± s.d. of three biological replicates. *p < 0.05, **p < 0.01 vs Scramble. (B) The percentages of beating EBs derived from scramble shRNA or shHIF2α ESCs. Data represent the mean ± s.d. of three biological replicates. **p < 0.01 vs Scramble. (C) qRT-PCR analysis of cardiomyocyte-associated gene (Gata4, Nkx2.5, Tbx5, Mesp1, Mef2C, α-MHC and cTnT) expression in shHIF2α ESC-derived EBs. Transcript levels were normalized against scramble shRNA controls. Data represent the mean ± s.d. of three biological replicates. *p < 0.05, **p < 0.01 vs Scramble.