Figure 1
From: HIF2α induces cardiomyogenesis via Wnt/β-catenin signaling in mouse embryonic stem cells
The effect of HIF2α on the self-renewal of mouse ESCs. (A) Western blot analysis of 46C mouse ESCs overexpressing empty vectors or Flag-tagged HIF2α. (B) Morphology of mouse ESCs transfected with empty or Flag-tagged HIF2α vectors and cultured in serum/LIF for five passages. Scale bar, 100 μm. (C) Cell growth assay. Relative cell proliferation was measured as a standard at Days 0, 2, 4 and 6. The original cell density was 3,000/well. Data represent the mean ± s.d. of three biological replicates. (D) Quantitative RT-PCR (qRT-PCR) analysis of Oct4, Sox2 and Nanog in mouse ESCs transfected empty- or Flag-tagged HIF2α vectors. Data represent the mean ± s.d. of three biological replicates.