Effect of xanthine oxidase inhibition by daily oral administration of allopurinol (20 mg.kg
, last 4 weeks) on 4-hydroxy-2-noneal Michael Adducts (4HNE), angiotensin II (AngII) and angiotensin receptor type 1 (AT
) immunofluorescence in heart (a, c and e respectively) and coronary artery (b, d and f respectively) sections from rats with high fructose high fat (for 12 weeks) induced insulin resistance (IR). Data are presented as mean ± standard error of 8 animals in each group. *P < 0.05, compared with the corresponding control group values; #P < 0.05 compared with the corresponding IR group values; by one way ANOVA and Newman Keuls’ post hoc test. Micrographs at the top are representative fluorescence images of heart and coronary artery cross sections immunofluorescence stained by 4HNE, AngII or AT1 antibodies followed by Alexa fluor conjugated secondary antibodies.