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Figure 6 | Journal of Translational Medicine

Figure 6

From: The long non-coding RNA HOTTIP promotes progression and gemcitabine resistance by regulating HOXA13 in pancreatic cancer

Figure 6

HOXA13 partly mediates the effect of HOTTIP on PDAC biology. (A) MIA PaCa-2 and SW1990 cells were transfected with control siRNA or siRNA against HOXA13, and HOXA13 expression was subsequently determined by qRT­PCR. (B) HOXA13 knockdown efficiency was also confirmed by western blotting. (C) (D) Cell viability of MIA PaCa-2 (C) and SW1990 cells (D) was determined at the indicated time points by CCK-8 assays. (E). Effect of HOXA13 on cell invasion ability was measured by Transwell assays. (F). The number of invading cells was analyzed. (G) (H) The expression levels of EMT-related genes (E-cadherin, Vimentin, and Snai1) was evaluated by qRT-PCR 48 h after transfection in both cell lines. (I) (J) The effect of HOXA13 silencing on E-cadherin, Vimentin, and Snail 1 protein levels was confirmed by western blotting. (K) Immunofluorescence staining for the EMT makers in SW1990 cells. Data represent the mean ± s.d. from three independent experiments. **p < 0.01, Student’s t-test.

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