HOXA13 partly mediates the effect of HOTTIP on PDAC biology. (A) MIA PaCa-2 and SW1990 cells were transfected with control siRNA or siRNA against HOXA13, and HOXA13 expression was subsequently determined by qRTPCR. (B) HOXA13 knockdown efficiency was also confirmed by western blotting. (C) (D) Cell viability of MIA PaCa-2 (C) and SW1990 cells (D) was determined at the indicated time points by CCK-8 assays. (E). Effect of HOXA13 on cell invasion ability was measured by Transwell assays. (F). The number of invading cells was analyzed. (G) (H) The expression levels of EMT-related genes (E-cadherin, Vimentin, and Snai1) was evaluated by qRT-PCR 48 h after transfection in both cell lines. (I) (J) The effect of HOXA13 silencing on E-cadherin, Vimentin, and Snail 1 protein levels was confirmed by western blotting. (K) Immunofluorescence staining for the EMT makers in SW1990 cells. Data represent the mean ± s.d. from three independent experiments. **p < 0.01, Student’s t-test.