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Figure 4 | Journal of Translational Medicine

Figure 4

From: The long non-coding RNA HOTTIP promotes progression and gemcitabine resistance by regulating HOXA13 in pancreatic cancer

Figure 4

HOTTIP knockdown enhances the chemosensitivity of human pancreatic cancer cells to gemcitabine in vitro and in vivo. (A) SW1990 cells were infected with control shRNA or an shRNA against HOTTIP as indicated, and then treated with gemcitabine (0.1, 1, or 10 μM) for 72 h. Cell survival rates were then measured using CCK-8 assays. The values are presented as the means ± s.d. **P < 0.01. (B) The effect of HOTTIP suppression on the IC50 of SW1990 cells was calculated. (C) SW1990 cells were infected with control shRNA or shRNA against HOTTIP as indicated and then treated with gemcitabine (10 μM) or PBS (100 μL) for 14 days. Colony formation on plastic was then assessed. Representative plates are shown. The number of colonies on each plate was calculated using Image J. The first group was set as the control group, and served as the baseline for colony number normalization. (D) SW1990 cells were transduced with control shRNA or shRNA against HOTTIP as indicated. Cells (3 × 105) were then subcutaneously injected into mice, and gemcitabine or PBS were injected intraperitoneally once every 3 days for five cycles. Representative images of tumor-bearing mice are shown. (E) Images of tumors from all mice in each group. (F) Tumor volumes were measured on the indicated days. (G) Tumor weights were determined. In vitro data are represented as the mean ± s.d. from three independent experiments. **p < 0.01, Student’s t-test.

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