HOTTIP knockdown inhibits PDAC cell invasion and epithelial-mesenchymal transition
(EMT). (A) MIA PaCa-2 and SW1990 cells were transduced with control shRNA or an shRNA against HOTTIP as indicated, and invasion assays were performed. Representative photos of invasive cells on the membrane are shown. (B) The effect of HOTTIP knockdown on the invasion ability of MIA PaCa-2 cells and SW1990 cells was quantified by counting the number invasive cells. (C) (D) The effect of HOTTIP knockdown on expression of E-cadherin, Vimentin, and Snail 1 was evaluated by qRT-PCR. (E) (F) The effect of HOTTIP-knockdown on E-cadherin, Vimentin, and Snail 1 protein levels was confirmed by western blotting. (G) (H) (I) Immunofluorescence staining for the EMT makers in SW1990 cells. Data represent the mean ± s.d. from three independent experiments. **p < 0.01, Student’s t-test.