Figure 2From: The long non-coding RNA HOTTIP promotes progression and gemcitabine resistance by regulating HOXA13 in pancreatic cancer HOTTIP knockdown inhibits cell growth and cell cycle in PDAC cells. (A) The efficiency of HOTTIP silencing in short hairpin RNA-stably transduced PDAC cell lines. Relative gene expression was determined using the comparative delta-delta CT method (2-∆∆Ct). (B) (C) MIA PaCa-2 and SW1990 cells were infected with control shRNA or an shRNA against HOTTIP as indicated. Cell viability was determined at the indicated time points after transfection using CCK-8 assays. (D) (E) MIA PaCa-2 and SW1990 cells were tansduced with control shRNA or an shRNA against HOTTIP as indicated. The cell-cycle distribution was assessed following transduction by flow cytometry. Data represent the mean ± s.d. from three independent experiments. **p < 0.01, Student’s t-test.Back to article page