Figure 4From: Mir-135a enhances cellular proliferation through post-transcriptionally regulating PHLPP2 and FOXO1 in human bladder cancer MiR-135a modulates expression of cell cycle regulators, including p21 Cip1 , p27 Kip1 , Cyclin D1 and Ki67. A. Western blotting analysis of p21Cip1, p27Kip1 , Cyclin D1 and Ki67 in indicated bladder cancer cells. α-Tubulin was used as a loading control. B. Real-time PCR analysis of p21 Cip1, p27 Kip1, Cyclin D1 and Ki67 mRNA in bladder cancer cells transfected with miR-135a or NC. C. Real-time PCR analysis of p21 Cip1 , p27 Kip1, Cyclin D1 and Ki67 mRNA in bladder cancer cells transfected with the miR-135a inhibitor or NC. GAPDH was used as a loading control. Bars represent the mean ± SD values of three independent experiments; *P < 0.05.Back to article page