Expression of miR-135a is elevated in bladder cancer cells. A. Real-time PCR analysis of miR-135a expression in bladder cancer cells, including primary culture cells of bladder cancer tissues (shown as T#1, T#2, T#3) and bladder cancer cell lines, EJ, T24, BIU87, SCaBER, and 5637, compared to normal bladder cells as controls. The normal control for bladder cancer cell lines are primary cultures of normal bladder epithelial cells established from fresh specimens of normal bladder tissues (shown as NC #1, #2, #3). B. The expression of miR-135a was examined in seven pairs of cancerous tissues (T) and their adjacent non-cancerous bladder tissues (ANT). The average miR-135a expression was normalized using U6 expression. Bars represent the mean ± SD of three independent experiments. **P <0.01, *P <0.05.