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Table 5 Calculation of total stem cell activity between the umbilical cord blood segment and unit based on the number of colonies and ATP concentrations at 2,000 cells/well

From: Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units

CFU assay

 

CFC-GEMM at 1 × 10 5 cells

HPP-SP at 1 × 10 5 cells

Total CFC-GEMM

Total HPP-SP

Colonies – TNC Segment

475 ± 96

ND

6,835 ± 1,374

ND

Colonies-MNC Segment

2,275 ± 433

ND

13,533 ± 2,005

ND

Colonies – TNC Unit

487.5 ± 165

ND

8,141 ± 2,759

ND

Colonies – MNC Unit

2,400 ± 248

ND

23,624 ± 1,617

ND

ATP Bioluminescence Proliferation Assay

 

CFC-GEMM at 1 × 105 cells

HPP-SP at 1 × 105 cells

Total CFC-GEMM in the Unit

Total HPP-SP in the Unit

ATP (μM) - TNC Segment

22.0 ± 15.1

24.1 ± 20.0

317 ± 217

347 ± 288

ATP (μM) – MNC Segment

58.5 ± 6.5

73.8 ± 2.4

494 ± 44

597 ± 16

ATP (μM) – TNC Unit

29.0 ± 8.6

33.9 ± 11.1

8,732 ± 2,572

10,180 ± 3,335

ATP (μM) – MNC Unit

88.2 ± 11.9

107.2 ± 11.5

13,679 ± 1,440

17,991 ± 1,396

  1. ND = not done.
  2. Statistics for CFU assay.
  3. TNC segment vs MNC segment: Colonies/105, P = 0.76; Total colonies, P = 0.17.
  4. MNC unit vs MNC unit: Colonies/105, P = 0.57; Total colonies, P = 0.013.
  5. Statistics for ATP assay.
  6. TNC segment vs MNC segment for CFC-GEMM: ATP/105, P = <0.0001; Total ATP, P = 0.024. For HPP-SP: ATP/105, P = <0.0001; Total ATP, P = 0.0032.
  7. TNC unit vs MNC unit for CFC-GEMM: ATP/105, P = <0.0001; Total ATP, P = 0.01. For HPP-SP, ATP/105, P = <0.0001; Total ATP, P = 0.032.