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Table 2 Calculation of total primitive hematopoietic stem cell (CFC-GEMM) activity based on the number of colonies and ATP concentrations at 2,500 cells

From: Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units

 

CFC-GEMM at 1 × 10 5 cells

Total CFC-GEMM

Colony number in TNC fraction

800 ± 242

37,840 ± 11,424

Colony number in MNC fraction

3,175 ± 300

49,085 ± 4,279

ATP (μM) in TNC fraction

8.8 ± 2.6

416 ± 125

ATP (μM)in MNC fraction

35.4 ± 3.3

673 ± 47

  1. Calculation of total CFC-GEMM was performed as described by Page et al. [10] and was based on a recovery of 29.03% after density gradient centrifugation. This recovery was similar to that obtained by Basford et al. [30].
  2. Statistics for CFU Assay.
  3. TNC fraction vs MNC fraction: CFU/105, P = <0.0001; Total CFU, P = 0.15.
  4. Statistics for ATP Assay.
  5. TNC fraction vs MNC fraction: ATP/105, P = <0.0001; Total ATP, P = 0.024.