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Figure 3 | Journal of Translational Medicine

Figure 3

From: Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units

Figure 3

Comparison Between Paired UCB Segments. The results represent the combined data from 12 segment pairs from different UCB lots. Segments were randomly allocated as Segment 1 and 2. For each segment, two stem cell populations were measured from both the unseparated, TNC and separated, MNC fractions. The results are provided as box and whisker plots for each cell dose (1,000 cells/well, clear box; 2,000 cells/well, light grey box; 4,000 cells/well, dark grey box) for a total of 12 paired segments. The top and bottom of the box represent the 25 and 75 percentiles. The horizontal line and “+” sign within the box represent the median and mean of the results, respectively. The whiskers (error bars) represent the 5 and 95% confidence intervals. The asterisk (*) represents results from a two-way ANOVA showing a statistical difference (P =< 0.05) of the respective cell dose compared to the CFC-GEMM, TNC, Segment 1 sample. The dotted horizontal lines at the bottom of the graph represent the acceptance/rejection levels ± 15% for cell proliferation measured by ATP bioluminescence.

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